Engineering biological devices that can emulate capabilities of living cells from molecular building blocks is a longstanding goal of synthetic biology. In recent years great progress has been made in recapitulating sophisticated cytosolic processes from purified components, such as the ability to regulate protein synthesis. In contrast, artificial cell membranes lag behind in their capabilities due to a poor understanding of how to engineer membrane complexity, for example, by introducing functional membrane proteins. Among the many outstanding challenges, one is the lack of precision techniques to systematically explore the multidimensional spaces of membrane composition parameters like lipid content. In consequence, relationships between critical membrane processes, e.g. protein binding and inserting, and lipid composition are poorly characterized. To alleviate this need, we present the development of suite of microfluidic tools to build, manipulate artificial cells with different membrane composition and quantify their biophysical properties at the single artificial cell level. Advances in our microfluidic tool-kit includes devices for on-the-fly variation of artificial cell lipid composition and multiplexed perfusion over immobilized artificial cell populations. Furthermore, we add to our compositional probes by developing a DNA based optical sensor for assaying membrane surface charge.