Exocytosis is a complex process involving the regulated release of neurotransmitters from presynaptic neurons, and precise control of this process is crucial for neurotransmission. Synapsin and the SNARE (Soluble NSF Attachment Protein Receptor) complex are proteins that play significant roles in regulating exocytosis. Studies have demonstrated that synapsin modulates vesicle release by controlling the movement of vesicles to the active zone, where the SNARE complex facilitates vesicle fusion with the presynaptic membrane. Despite synapsin being the most abundant protein in neurons and both proteins interacting with synaptic vesicles, the role of synapsin in modulating SNARE dynamics remains unclear. In this investigation, we employed magnetic tweezers to probe the interaction between synapsin and the SNARE complex. By exerting controlled forces on individual SNARE complexes in the presence of synapsin, we observed that synapsin can impact the mechanical properties of SNARE, implying a potential role for synapsin in regulating neurotransmitter release through its effects on SNARE dynamics. These findings emphasize the importance of exploring synaspin-SNARE interactions in the nervous system and offer fresh insights into the role of synapsin in neuronal function.